Composite

Part:BBa_K3711055

Designed by: Jiacheng Shi   Group: iGEM21_HUST-China   (2021-10-03)


Pynr071c-α factor-Laccase-AOX1 Terminator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2353
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This is a composite part for extracellular expression of Laccase. Laccase is expressed and participates in the production from indigo to colorless substance. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express laccase outside of the cell.

Usage and Biology

Laccase has a wide variation of substrates, which includes phenol, aniline, carboxylic acid (and their derivatives), bio-pigments, lignans, organic metal compounds and other non-phenol compounds. The optimum temperature of laccase is relatively low, while it has the maximum catalyzing efficient among environment of low pH. These characters give laccase a promising prospect in food industry, paper industry, textile industry and bio-repairing of soil. Coriolus versicolor from Aphyllophorales, Aphyllophorales, Hymenomycetes, Basidiomycota, is one of the ideal strains which is used for laccase production and have excellent degradation ability to lignans. Laccase in plants is involved in various botanic metabolism pathways, such as synthesis of lignans, synthesis and degradation of pigments and elongation of roots.
Copper atom is usually contained in the laccase, which is one of the common characters of laccase and maintains its oxidizing/reducing reaction. As the key element of reaction center, copper atom is critical to the activity and specificity of laccase since it exists in the area that binds substrate and breaks & forms chemical bonds. According to crystal structure of laccase, most have 3 copper binding sites which bind 4 copper atoms.

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